The liver is involved in a wide range of activities in vertebrates and some other animals,
including metabolism, protein synthesis, detoxification, and the immune system. Until now,
various methods have been devised to study liver diseases; however, each method has its
own limitations. In situ liver perfusion machinery, originally developed in rats, has been
successfully adapted to mice, enabling the study of liver diseases. Here we describe the
protocol, which is a simple but widely applicable method for investigating the liver diseases …