Introduction: Healthcare workers (HCWs) are daily exposed to hepatitis B virus (HBV) infections from the blood and body fluids of patients and clients they care for. Unfortunately, many of these HCWs are not vaccinated for hepatitis B. The aim of this study is to assess the factors influencing knowledge of hepatitis B vaccination amongst HCWs in a tertiary health facility located in a rural community in Edo State, Southern Nigeria. Methods: A descriptive cross-sectional study design was used in this study, and data were collected from health workers randomly selected using the multistage sampling technique. 280 HCWs were administered with pretested, semi-structured interviewer-administered questionnaires. Data analysis was done using the Chi-square test (and Fisher Exact where appropriate) with the aid of IBM SPSS version 21.0 software, and the level of significance–alpha (α) was set at 5%(0.05).

Results: The mean age of HCWs in this study was 34.90 (±9.46) years. Just above half of the HCWs, 146 (52.1%), had a good knowledge of hepatitis B (HB) vaccine. Work type (p< 0.001) and category of HCWs (p< 0.001) were significantly associated with the level of knowledge of hepatitis B vaccination by respondents. Conclusion: There was poor knowledge of hepatitis B vaccination amongst HCWs with work type and category of HCW as determinants. Priority should be given to ensure targeted health promotion programmes that will improve knowledge, with a view to enhance optimal uptake of hepatitis B vaccine amongst HCWs.