Ferric species of the giant extracellular hemoglobin of Glossoscolex paulistus as function of pH: An EPR study on the irreversibility of the heme transitions

LM Moreira, AL Poli, JP Lyon, J Saade… - … and Physiology Part B …, 2008 - Elsevier
The present article is focused on the transitions of ferric heme species of the giant
extracellular hemoglobin of Glossoscolex paulistus (HbGp) induced by successive
alterations in pH, involving alkaline and acid mediums. Electron paramagnetic resonance
(EPR) is the spectroscopy used to evaluate the transitions that occur in the first coordination
sphere of ferric ion as a consequence of ligand changes in a wide range of pH, since this
tool is very sensitive to slight changes that occur in the heme pocket of paramagnetic …
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