Foundations of the use of an enzyme-kinetic analogy in cell-mediated cytotoxicity
SJ Merrill - Mathematical Biosciences, 1982 - Elsevier
A mathematical model of the 51 Cr-release microcytotoxicity assay is utilized to find
conditions under which the kinetics of this assay resemble the kinetics of a classical enzyme-
substrate reaction. Assuming a steady-state approximation, that “bystander” effector cells do
not bind markedly better than the cytotoxic effector cells, and that the programming of the
target cells for lysis is irreversible, it is shown that the velocity of label release is v= v max
TT/(K 1 2+ TT), where both V max and K 1 2 are linear functions of the effector-cell …
conditions under which the kinetics of this assay resemble the kinetics of a classical enzyme-
substrate reaction. Assuming a steady-state approximation, that “bystander” effector cells do
not bind markedly better than the cytotoxic effector cells, and that the programming of the
target cells for lysis is irreversible, it is shown that the velocity of label release is v= v max
TT/(K 1 2+ TT), where both V max and K 1 2 are linear functions of the effector-cell …
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