Genetic Transformation in Citrus

D Donmez, O Simsek, T Izgu… - The Scientific World …, 2013 - Wiley Online Library
… In order to carry out successful gene transformation studies in citrus, optimized in …
transformation studies. There are also many efficient regeneration protocols published in different …

Genetic transformation of Citrus protoplasts and regeneration of transgenic plants

A Vardi, S Bleichman, D Aviv - Plant Science, 1990 - Elsevier
… Figure 1 shows an example of a CAT assay performed 3 days after PEG-mediated
transformation. To test for stable transformation we focussed on the second gene (nptII) of plasmid …

Early Events in Agrobacterium‐mediated Genetic Transformation of Citrus Explants

L Pena, RM Perez, M Cervera, JA Juarez… - Annals of …, 2004 - academic.oup.com
Genetic transformation has been achieved for several citrus genotypes, including sweet orange
(Citrus sinensis… However, transformation efficiencies are generally low, and several other …

Evaluation of selection strategies alternative to nptII in genetic transformation of citrus

A Ballester, M Cervera, L Pena - Plant cell reports, 2008 - Springer
citrus transformation; however, it may be recommendable to replace it given the pressure
exerted against antibiotic-resistance selectable marker genes … in two citrus genotypes, Carrizo …

[HTML][HTML] Citrus genetic transformation: an overview of the current strategies and insights on the new emerging technologies

G Conti, B Xoconostle-Cázares… - Frontiers in Plant …, 2021 - frontiersin.org
… technical aspects of citrus genetic transformation procedures via … citrus genetic transformation.
This review also explores alternative emerging transformation strategies applied to citrus

Genetic transformation of lime (Citrus aurantifolia Swing.): factors affecting transformation and regeneration

L Pena, M Cervera, J Juárez, A Navarro, JA Pina… - Plant Cell Reports, 1997 - Springer
… , a genetic transformation system for lime could be very valuable not only for improvement of
this crop, but also as a citrus model … of resistance against CTV through genetic engineering. …

[图书][B] Citrus transformation: challenges and prospects

V Febres, L Fisher, A Khalaf, GA Moore - 2011 - intechopen.com
… annotations, additional functional analyses are crucial to study the nature of the expected
phenotype before we can introduce new genes into the Citrus genome using transformation

Genetic transformation and genes for resistance to abiotic and biotic stresses in Citrus and its related genera

XQ Gong, JH Liu - Plant Cell, Tissue and Organ Culture (PCTOC), 2013 - Springer
gene cloning and genetic transformation may avoid genetic segregation and keep the desirable
horticultural traits in commercial citrusgene cloning and genetic transformation in Citrus

Genetic transformation and plant recovery from mature tissues of Citrus sinensis L. Osbeck

WAB Almeida, FAA Mourão Filho, LE Pino… - Plant Science, 2003 - Elsevier
Genetic transformation using mature tissue as explant is important for the precocious evaluation
of the genetically … and genetic transformation of sweet orange varieties (Citrus sinensis L…

Genetic transformation of Citrus sinensis cv. Hamlin with hrpN gene from Erwinia amylovora and evaluation of the transgenic lines for resistance to citrus canker

JM Barbosa-Mendes, FAA Mourão Filho… - Scientia …, 2009 - Elsevier
… These facts stimulated our interest in the use of the hrpN gene for citrus genetic transformation.
In our study, transgenic citrus plants expressing hrpN gene showed increased resistance …