This paper is devoted to the tunneling radiation and quantum gravity effect on tunneling radiation of neutral regular black hole in Rastall gravity. We analyzed the tunneling radiation and Hawking temperature of neutral regular black hole by applying the Hamilton-Jacobi ansatz phenomenon. Lagrangian wave equation have been investigated by generalized uncertainty principle (GUP), using the WKB-approximation and calculated the tunneling rate as well as temperature. Furthermore, we analyzed the temperature of this neutral regular black hole in the presence of gravity. The stability and instability of neutral regular black hole are also analyzed.