High-efficiency thermal asymmetric interlaced PCR for amplification of unknown flanking sequences

YG Liu, Y Chen - Biotechniques, 2007 - Taylor & Francis
… sizes, we developed a high-efficiency TAIL-PCR (hiTAIL-PCR) … as determined by sequencing
of primary hiTAIL-PCR products … and suppression-PCR, thus greatly increased the success …

High-efficiency thermal asymmetric interlaced PCR (hiTAIL-PCR) for determination of a highly degenerated prophage WO genome in a Wolbachia strain infecting a fig …

GH Wang, JH Xiao, TL Xiong, Z Li… - Applied and …, 2013 - Am Soc Microbiol
… , TAIL-PCR allows the … high-efficiency TAIL-PCR (hiTAIL-PCR), increase both the rate of
success to >90% and target products sizes to 1 to 3 kb (14). We used hiTAIL-PCR to determine

[PDF][PDF] High-Efficiency Thermal Asymmetric

RW Murphy, DW Huang, GH Wang, JH Xiao, TL Xiong… - 2013 - researchgate.net
… is highly degenerated … high-efficiency TAIL-PCR (hiTAIL-PCR), increase both the rate of
success to 90% and target products sizes to 1 to 3 kb (14). We used hiTAIL-PCR to determine

[PDF][PDF] Randomly primed and ligation-independent PCR methods: High efficiency and cost effective strategies for genome walking and flanking sequence cloning

Z Wang, GQ Liu, MZ Bao, GG Ning - pdfs.semanticscholar.org
determine the full gene structure26,27. Whereas, the improved … SiteFinding-PCR, hiTAIL-PCR,
SEFA-PCR and FPNI-PCR, all of … A large number of experiments using FPNI-PCR greatly

TADEA‐PCR is a highly efficient method of amplifying unknown flanking fragments of T‐DNA transformants

Y Yang, Y Cao, HQ Xu, L Gao, X Guo… - Physiologia …, 2018 - Wiley Online Library
… Next, we determined whether TADEA-PCR worked effectively … The only difference is that
hiTAIL-PCR uses an arbitrary … Its high efficiency results from the applications of TADEs. The …

A method for amplification of unknown flanking sequences based on touchdown PCR and suppression-PCR

S Gao, D He, G Li, Y Zhang, H Lv, L Wang - Analytical Biochemistry, 2016 - Elsevier
… The hiTAIL-PCR method was used as the control in this study and … This greatly reduces the
experimental cost, labor and time. … , the cost of flanking sequence analysis of each sample is …

Fusion primer and nested integrated PCR (FPNI-PCR): a new high-efficiency strategy for rapid chromosome walking or flanking sequence cloning

Z Wang, S Ye, J Li, B Zheng, M Bao, G Ning - BMC biotechnology, 2011 - Springer
… integrated PCR method (FPNI-PCR) for the identification and … The hiTAIL-PCR technique
also poses a great challenge to … TAIL-PCR and FPNI-PCR methods demand only extremely

High-efficiency thermal asymmetric interlaced PCR (hiTAIL-PCR) for determination of a highly degenerated prophage WO genome in a Wolbachia strain infecting a …

WGH Wang GuanHong, XJH Xiao JinHua… - 2013 - cabidigitallibrary.org
… We determine the complete genome sequence of prophage WO (WOSol) in Wolbachia …
), by high-efficiency thermal asymmetric interlaced PCR. The genome of WOSol is highly

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production by Haloarchaeon Halogranum amylolyticum

YX Zhao, ZM Rao, YF Xue, P Gong, YZ Ji… - Applied microbiology and …, 2015 - Springer
high-efficiency thermal asymmetric interlaced (hiTAIL) PCR … of microbial contamination
could be greatly reduced. Thirdly, … , we then designed primers for hiTAIL PCR for acquiring the …

Analysis of Integrated Structure of the Exogenous Gene Insertion and the Establishment of Event-specific Detection Method in Transgenic Insect Resistant Cotton

N Hou, H He, M Dong, R Xu, Y Wan… - GMO Biosafety …, 2012 - ecoevopublisher.com
… '-terminal high-efficiency thermal asymmetric interlaced PCR (… In this study, Using hiTAIL-PCR
and long-chain PCR method, … qualitative PCR primer screened in this study had very good …