High-throughput SARS-CoV-2 and host genome sequencing from single nasopharyngeal swabs

JE Gorzynski, HN De Jong, D Amar, C Hughes… - medRxiv, 2020 - medrxiv.org
During COVID19 and other viral pandemics, rapid generation of host and pathogen genomic
data is critical to tracking infection and informing therapies. There is an urgent need for
efficient approaches to this data generation at scale. We have developed a scalable, high
throughput approach to generate high fidelity low pass whole genome and HLA sequencing,
viral genomes, and representation of human transcriptome from single nasopharyngeal
swabs of COVID19 patients.
Abstract
During COVID19 and other viral pandemics, rapid generation of host and pathogen genomic data is critical to tracking infection and informing therapies. There is an urgent need for efficient approaches to this data generation at scale. We have developed a scalable, high throughput approach to generate high fidelity low pass whole genome and HLA sequencing, viral genomes, and representation of human transcriptome from single nasopharyngeal swabs of COVID19 patients.
medrxiv.org
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