High-yield expression and purification of isotopically labeled norcoclaurine synthase, a Bet v 1-homologous enzyme, from Thalictrum flavum for NMR studies

H Berkner, J Engelhorn, DK Liscombe… - Protein expression and …, 2007 - Elsevier
The enzyme norcoclaurine synthase (NCS) found in the common meadow rue, Thalictrum
flavum, and other plants shows sequence homology to members of the class 10 of
pathogenesis related (PR 10) proteins that contains allergens such as the major birch pollen
allergen Bet v 1, the major cherry allergen Pru av 1, and the major apple allergen Mal d 1.
The enzyme is involved in the plant's secondary metabolism and is required for the
production of bioactive secondary metabolites like morphine. Whereas the physiological …
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