Many life cycle assessment (LCA) studies comparing environmental impacts of different beef production systems are incomplete as they exclude biodiversity impacts and soil carbon stock changes. This study aims to assess the environmental impact of ruminant production on semi-natural grasslands or so-called high nature value (HNV) farms at the European level. We collected data for 24 HNV farms in Europe (in Finland, Estonia and France). The studied farms are extensive beef, sheep and goat production systems. We used LCA to assess the potential environmental impact of HNV farms according to global warming potential (GWP100), eutrophication, fossil fuels and water use, by using the Solagro carbon calculator and OpenLCA software. Results showed that HNV farming systems have the potential to maintain unique biodiversity, act as carbon sinks, reduce greenhouse gas emissions and reduce nutrient losses and water use while producing animal-derived food. There were significant differences between HNV farms among countries in their greenhouse gas emissions at the farm level (tCO2eq ha-1) and N inputs (kg N ha-1). Better regional understanding of the environmental impact performance of HNV farming systems