Hydrodynamic radii of ranibizumab, aflibercept and bevacizumab measured by time-resolved phosphorescence anisotropy
LM Hirvonen, GO Fruhwirth, N Srikantha… - Pharmaceutical …, 2016 - Springer
Pharmaceutical Research, 2016•Springer
Purpose To measure the hydrodynamic radii of intravitreal anti-VEGF drugs ranibizumab,
aflibercept and bevacizumab with μ s time-resolved phosphorescence anisotropy. Methods
Ruthenium-based dye Ru (bpy) 2 (mcbpy− O− Su− ester)(PF 6) 2, whose lifetime of several
hundred nanoseconds is comparable to the rotational correlation time of these drugs in
buffer, was used as a label. The hydrodynamic radii were calculated from the rotational
correlation times of the Ru (bpy) 2 (mcbpy− O− Su− ester)(PF 6) 2-labelled drugs obtained …
aflibercept and bevacizumab with μ s time-resolved phosphorescence anisotropy. Methods
Ruthenium-based dye Ru (bpy) 2 (mcbpy− O− Su− ester)(PF 6) 2, whose lifetime of several
hundred nanoseconds is comparable to the rotational correlation time of these drugs in
buffer, was used as a label. The hydrodynamic radii were calculated from the rotational
correlation times of the Ru (bpy) 2 (mcbpy− O− Su− ester)(PF 6) 2-labelled drugs obtained …
Purpose
To measure the hydrodynamic radii of intravitreal anti-VEGF drugs ranibizumab, aflibercept and bevacizumab with μs time-resolved phosphorescence anisotropy.
Methods
Ruthenium-based dye Ru(bpy)2(mcbpy − O − Su − ester)(PF6)2, whose lifetime of several hundred nanoseconds is comparable to the rotational correlation time of these drugs in buffer, was used as a label. The hydrodynamic radii were calculated from the rotational correlation times of the Ru(bpy)2(mcbpy − O − Su − ester)(PF6)2-labelled drugs obtained with time-resolved phosphorescence anisotropy measurements in buffer/glycerol solutions of varying viscosity.
Results
The measured radii of 2.76±0.04 nm for ranibizumab, 3.70±0.03 nm for aflibercept and 4.58±0.01 nm for bevacizumab agree with calculations based on molecular weight and other experimental measurements.
Conclusions
Time-resolved phosphorescence anisotropy is a relatively simple and straightforward method that allows experimental measurement of the hydrodynamic radius of individual proteins, and is superior to theoretical calculations which cannot give the required accuracy for a particular protein.
Springer
以上显示的是最相近的搜索结果。 查看全部搜索结果