The aim of the research was to analyze effects of the gene Gpc-B1 , introgressed from wild spelt Triticum turgidum ssp. dicoccoides , on the presence of biologically important elements in bread winter wheat hybrid families of generations F4 and F5. The accumulation of metals in ripe and unripe grains was measured on a mass spectrometer with inductively coupled plasma ICP-MS Agilent 7700x. It was found that the expressing gene significantly increased the content of trace elements Fe, Mn, Zn and Cu in ripe wheat kernels on average by 50-70%, while the increase of secondary nutrients or mesoelements Mg, Ca defined by an average of 20-40%. The enrichment of minerals, confirmed during grain development and ripening provides not only biofortification for the future harvest, but also potentially enhances the resistance of plants to diseases and formation of seedlings with more efficient use of nitrogen.