This research aims to examine the impact of training, mood, and motivation on employee performance in academia of Sindh province, Pakistan. Training plays a vital role in enhancing the skills, abilities, and confidence of employees to achieve their personal and organizational goals effectively. Moreover, the mood and motivation of employees are also considered necessary features for the performance of employees. Therefore, an employee performance survey was conducted by adopting a close-ended questionnaire based on a 5-points and 7-points Likert scale to identify the relationship and impact on the performance of faculty. The method of sampling was convenience sampling, which enabled us to reach 265 participants from four cities of Sindh, including Karachi, Sukker, Hyderabad, and Mirpurkhas. SPSS was used to analyze the data, which highlights that training and motivation have a strong positive relationship to the dependent variable i: e employee performance; mood and employee performance have a moderately positive relationship. Every competitive organization in today's world needs a skilled workforce to achieve its short- and long-term organizational goals, so it is the organization's responsibility.