Improved diagnosis of pulmonary tuberculosis by detection of antibodies against multiple Mycobacterium tuberculosis antigens
A Raja, UD Ranganathan, R Bethunaickan - Diagnostic microbiology and …, 2008 - Elsevier
Two secreted antigens (38 and 30 kDa) and 1 cytosolic antigen (16 kDa) were purified in our
laboratory from Mycobacterium tuberculosis culture filtrate and cytosol using
chromatographic/electrophoretic methods. One recombinant antigen (27 kDa, MPT51)
expressed in Escherichia coli was also isolated. All the 4 antigens were tested individually
for detection of serum IgG, IgA, and IgM (a total of 476 sera from 5 groups) by indirect
enzyme-linked immunosorbent assay. Keeping the well-reported 38 kDa as the main …
laboratory from Mycobacterium tuberculosis culture filtrate and cytosol using
chromatographic/electrophoretic methods. One recombinant antigen (27 kDa, MPT51)
expressed in Escherichia coli was also isolated. All the 4 antigens were tested individually
for detection of serum IgG, IgA, and IgM (a total of 476 sera from 5 groups) by indirect
enzyme-linked immunosorbent assay. Keeping the well-reported 38 kDa as the main …
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