[PDF][PDF] In silico oligonucleotide primer design for Campylobacter jejuni cytolethal distending toxin B gene amplification
RK Praja - Oceana Biomedicina Journal, 2021 - scholar.archive.org
Cytolethal distending toxin B (cdtB) is a genotoxin expressed by Campylobacter jejuni. cdtB
is a DNase that induces DNA double-strand breaks (DSB) in the nucleus causing cell cycle
arrest at the G2/M phase and apoptosis. This study aimed to design and analyze in silico
PCR primer pairs to amplify cdtB gene of C. jejuni. Sequence of cdtB gene with accession
number AY445094. 1 was retrieved from GenBank NCBI and primer pairs were designed by
using Primer-BLAST. Further analysis of primer quality related to self dimer, hairpin, repeat …
is a DNase that induces DNA double-strand breaks (DSB) in the nucleus causing cell cycle
arrest at the G2/M phase and apoptosis. This study aimed to design and analyze in silico
PCR primer pairs to amplify cdtB gene of C. jejuni. Sequence of cdtB gene with accession
number AY445094. 1 was retrieved from GenBank NCBI and primer pairs were designed by
using Primer-BLAST. Further analysis of primer quality related to self dimer, hairpin, repeat …
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