OBJECTIVE: To assess the effectiveness of the three-dimensional culture of spermatogenic
cells in a collagen gel matrix from nonobstructive azoospermic patients and examine the
relation between the success rate of in vitro spermatogenesis and serum FSH level as a
diagnostic prediction. DESIGN: Prospective study using radioimmunoassay,
immunocytochemistry, and flow cytometry with primary cultured cells. SETTING: Gynecologic
clinics and human reproduction research laboratory. PATIENT (S): Primary culture of …