We investigated roots of Cephalaria kotschyi Boiss. and Hoh.(Dipsacaceae) collected in September 2005 in Lerik Region in southern Azerbaijan [1]. Raw material was extracted three times with methanol (80%) by refluxing for 1 h. Solvent was distilled off. The solid (22 g) was diluted with water. The aqueous solution of the extract was extracted three times with butanol. The butanol extract (2 g) was purified by ordinary liquid chromatography over an RP18 column with a gradient of H2O: MeOH (70-30 v/v→ 0-100 v/v) as the mobile phase. This produced 24 fractions Rp1F1-Rp1F24. Then fraction Rp1F12 was purified by column chromatography over silica gel with elution by CH2Cl2: MeOH: H2O (40: 10: 1). This isolated 1 (5.4 mg). Fractionation of the butanol extract in a layer of polyamide using MeOH: H2O (10%→ 95% MeOH) gave six pure fractions Po1RM102-4-Po1RM95. Then fraction Po1RM102-4 was purified over silica gel using CH2Cl2: MeOH: H2O (40: 12: 2) to isolate 1 (18 mg) and 2 (20 mg). The chemical structures of the isolated compounds were determined by NMR. The NMR spectra of CD3OD solutions of the compounds were recorded on a Bruker DRX Avance 200 MHz spectrometer (1H 200 MHz, 13C 50 MHz)(Table 1).