The studied lactic acid bacteria (strains LRV 1, LRV 2, SP, S 1 and S 2 respectively) are part of a set of 50 strains newly isolated from calf ruminal liquid, pig stomach and silage. They are Grampositive, catalase negative, facultative anaerobic, non-motile and non-spore forming rods (LRV 1, SP, S 1) or cocci (LRV 2 and S 2). Optimal growth temperature occurred between 280C to 42 0C (strain dependent) into a pH range between 3.0 to 8.0 and up to 0.5% NaCl. The pattern of fermented carbohydrates on API 50 CHL galleries showed a diversity of fermentative metabolism for isolates LRV 1, SP and S 1. Testing with API 20 STREP showed that LRV 2 fermented raffinose and hydrolyzed hippuric acid while S 2 did not ferment raffinose and hippuric acid. 16S rDNA RFLP analysis confirmed similarity between strains belonging to the same species therefore supporting inclusion of the conspecific strains into the same OTU. Polyphasic identification showed that LRV 1, SP and S 1 represented three different strains of Lactobacillus plantarum subsequently denominated as Lactobacillus plantarum CMGB 1, Lactobacillus plantarum CMGB 2 and Lactobacillus plantarum CMGB 3 while LRV 2 and S 2 were two different strains of Enterococcus faecium that were subsequently denominated as Enterococcus faecium CMGB 6 and Enterococcus faecium CMGB 8.