Isolation of antibody V (D) J sequences from single cell sorted rhesus macaque B cells
C Sundling, G Phad, I Douagi, M Navis… - Journal of …, 2012 - Elsevier
Studies in nonhuman primates offer information of high relevance to clinical medicine due to
their close genetic relationship with humans. Here, we established an optimized protocol for
the isolation of antibody V (D) J sequences from rhesus macaque B cells. Nested PCR
primers were designed to align to sequences flanking the V (D) J coding region to enable
amplification of highly mutated antibody sequences. The primers were evaluated using
cDNA from bulk PBMCs as well as from single-sorted memory and naïve B cells from several …
their close genetic relationship with humans. Here, we established an optimized protocol for
the isolation of antibody V (D) J sequences from rhesus macaque B cells. Nested PCR
primers were designed to align to sequences flanking the V (D) J coding region to enable
amplification of highly mutated antibody sequences. The primers were evaluated using
cDNA from bulk PBMCs as well as from single-sorted memory and naïve B cells from several …
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