Isolation of commensal Escherichia coli strains from feces of healthy laboratory mice or rats
T Ju, BP Willing - Bio-protocol, 2018 - bio-protocol.org
The colonization abundance of commensal E. coli in the gastrointestinal tract of healthy
laboratory mice and rats ranges from 10 4 to 10 6 CFU/g feces. Although very well
characterized, the family that E. coli belongs to has a very homogeneous 16S rRNA gene
sequence, making the identification from 16S rRNA sequencing difficult. This protocol
provides a procedure of isolating and identifying commensal E. coli strains from a healthy
laboratory mouse or rat feces. The method can be applied to isolate commensal E. coli from …
laboratory mice and rats ranges from 10 4 to 10 6 CFU/g feces. Although very well
characterized, the family that E. coli belongs to has a very homogeneous 16S rRNA gene
sequence, making the identification from 16S rRNA sequencing difficult. This protocol
provides a procedure of isolating and identifying commensal E. coli strains from a healthy
laboratory mouse or rat feces. The method can be applied to isolate commensal E. coli from …
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