Key residues in subsite F play a critical role in the activity of Pseudomonas fluorescens subspecies cellulosa xylanase A against xylooligosaccharides but not against …
SJ Charnock, JH Lakey, R Virden, N Hughes… - Journal of Biological …, 1997 - ASBMB
In a previous study crystals of Pseudomonas fluorescens subspecies cellulosa xylanase A
(XYLA) containing xylopentaose revealed that the terminal nonreducing end glycosidic bond
of the oligosaccharide was adjacent to the catalytic residues of the enzyme, suggesting that
the xylanase may have an exo-mode of action. However, a cluster of conserved residues in
the substrate binding cleft indicated the presence of an additional subsite, designated
subsite F. Analysis of the biochemical properties of XYLA revealed that the enzyme was a …
(XYLA) containing xylopentaose revealed that the terminal nonreducing end glycosidic bond
of the oligosaccharide was adjacent to the catalytic residues of the enzyme, suggesting that
the xylanase may have an exo-mode of action. However, a cluster of conserved residues in
the substrate binding cleft indicated the presence of an additional subsite, designated
subsite F. Analysis of the biochemical properties of XYLA revealed that the enzyme was a …
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