Label-free oligonucleotide biosensor based on dual-peak long period fiber grating
X Chen, C Liu, MR Cardoso… - Optical Fibers and …, 2016 - spiedigitallibrary.org
Optical Fibers and Sensors for Medical Diagnostics and Treatment …, 2016•spiedigitallibrary.org
We report the simplification and development of biofunctionalization methodology based on
one-step 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-mediated reaction. The
dual-peak long period grating (dLPG) has been demonstrated its inherent ultrahigh
sensitivity to refractive index (RI), achieving 50-fold improvement in RI sensitivity over a
standard LPG sensor used in low RI range. With the simple and efficient immobilization of
unmodified oligonucleotides on sensor surface, dLPG-based biosensor has been used to …
one-step 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-mediated reaction. The
dual-peak long period grating (dLPG) has been demonstrated its inherent ultrahigh
sensitivity to refractive index (RI), achieving 50-fold improvement in RI sensitivity over a
standard LPG sensor used in low RI range. With the simple and efficient immobilization of
unmodified oligonucleotides on sensor surface, dLPG-based biosensor has been used to …
We report the simplification and development of biofunctionalization methodology based on one-step 1-ethyl-3-(3- dimethylaminopropyl)carbodiimide (EDC)-mediated reaction. The dual-peak long period grating (dLPG) has been demonstrated its inherent ultrahigh sensitivity to refractive index (RI), achieving 50-fold improvement in RI sensitivity over a standard LPG sensor used in low RI range. With the simple and efficient immobilization of unmodified oligonucleotides on sensor surface, dLPG-based biosensor has been used to monitor the hybridization of complementary oligonucleotides showing a detectable oligonucleotide concentration of 4 nM with the advantages of label-free, real-time, and ultrahigh sensitivity.
SPIE Digital Library以上显示的是最相近的搜索结果。 查看全部搜索结果