Dengue Hemorrhagic Fever (DHF) is still a health problem in the world including Indonesia. The number of dengue fever cases continues to increase in Gianyar Regency, Bali. This is not separated from the role of housewives in taking care of the house. The purpose of this research is to know the level of knowledge, attitude and behavior of housewife about mosquito nests eradication in Gianyar Regency in 2017. This research is a descriptive research conducted by using cross sectional method. The sample of this study is 106 housewives selected by multistage random sampling method. The results of this research indicates that the knowledge rates of housewives about mosquito nests eradication is enough 50.9%, less 29.2%, and good 19.8%. The result of housewife’s attitude shows enough attitude at 75.5%, good 20.8% and less 3.8%. For the results of housewife’s behavior reveals enough behavior 58.5%, less 34.9%, and good 6.6%. The results showed that the knowledge level, attitude, and behavior of housewives in the Batubulan Village, Sukawati District, Gianyar Regency about mosquito nests eradication reveals enough. Therefore, counseling about mosquito nests eradication activities should be done to improve knowledge, attitude and behavior of housewives.