The dispersed gene family 1 (DGF-1) is the fifth largest gene family in the T rypanosoma
cruzi genome, with over 500 members (11). Many of the predicted DGF-1 protein products
have several transmembrane domains and N-glycosylation and phosphorylation sites and
were thought to localize in the plasma membrane. Here, we report that affinity-purified
antibodies against a region of one of these proteins (DGF-1.2) localized it intracellularly in
different stages of the parasite. DGF-1.2 is more abundant in the amastigote stage than in …

MATERIALS AND METHODS Materials. Anti-Trypanosoma brucei pyruvate phosphate
dikinase (TbPPDK)-producing mouse hybridoma culture supernatant was a gift from
Frederique Bringaud (University of Bordeaux, Bordeaux, France); anti-T. brucei vacuolar
pyrophosphatase (TbVP1) was a gift from Norbert Bakalara (École Nationale Supérieure de
Chimie de Montpellier, France); anti-T. cruzi serine carboxypeptidase (TcSCP) was a gift
from Vanina Álvarez (University of General San Martín, Argentina). Alexa Fluor 488 …