Locating a Protein−Protein Interaction in Living Cells via Split Renilla Luciferase Complementation

A Kaihara, Y Kawai, M Sato, T Ozawa… - Analytical …, 2003 - ACS Publications
For spatial and quantitative kinetic analysis of protein− protein interactions (PPIs) in living
mammalian cells, a method was developed in which PPI-induced complementation of split
Renilla luciferase triggers spontaneous emission of luminescence using a cell membrane
permeable substrate, coelenterazine. This split Renilla luciferase complementation readout
was shown to work for locating a PPI between the tyrosine-phosphorylated peptide (Y941) of
IRS-1 and the SH2 domain of PI3K among insulin signaling pathways in living Chinese …
被引用次数：141 相关文章 所有 10 个版本

[引用][C] FLASHING A PROTEIN-PROTEIN INTERACTION IN LIVING CELLS VIA SPLIT RENILLA LUCIFERASE COMPLEMENTATION

Y Umezawa, A Kaihara - Bioluminescence and Chemiluminescence, 2005 - cir.nii.ac.jp
FLASHING A PROTEIN-PROTEIN INTERACTION IN LIVING CELLS VIA SPLIT <i>RENILLA</i>
LUCIFERASE COMPLEMENTATION | CiNii Research … FLASHING A PROTEIN-PROTEIN
INTERACTION IN LIVING CELLS VIA SPLIT <i>RENILLA</i> LUCIFERASE
COMPLEMENTATION …
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