Location of BRCA1 in human breast and ovarian cancer cells
123. ffECHNICALL COMMENTS antibodies depends on special cell fixation conditions, we
tested the subcellular localization of BRCA1 by biochemical extraction analysis in unfixed
cells. Three cancer cell lines (MCF7, SKOV-3, and U20S), each characterized by dotlike
nuclear staining and the absence of cytoplasmic staining after neutral paraformaldehyde
fixation, were analyzed by cell fractionation and immunoblotting for BRCA1 (Fig. 3). In all
three of these lines, BRCA1 was concentrated in the nuclear fraction. The validity of the …
tested the subcellular localization of BRCA1 by biochemical extraction analysis in unfixed
cells. Three cancer cell lines (MCF7, SKOV-3, and U20S), each characterized by dotlike
nuclear staining and the absence of cytoplasmic staining after neutral paraformaldehyde
fixation, were analyzed by cell fractionation and immunoblotting for BRCA1 (Fig. 3). In all
three of these lines, BRCA1 was concentrated in the nuclear fraction. The validity of the …
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