The present study was aimed to get extraction of natural colorants from golden duranta (Duranta repnes) leaves powder and its characterisation under microwave treatment. Dyeing powder and cotton fabrics, both were exposed to microwaves for 1, 2, 4, 6, 8 and 10 min. Aqueous, alkaline and methanol solubilised extracts were obtained from non-irradiated and irradiated golden duranta leaves powders. Chromium, tannic acid and pomegranate peel were applied to enhance colour strength of extracted dye. Golden duranta leaves powder and cotton fabrics treated with radiation significantly enhanced the extraction and dyeing process by reducing salt concentration. Microwave treatments also had significant affect on colour strength and fastness characteristics of cotton fabricss dyed with golden duranta. Microwave treatment of 1 min was the most affective dose for colorant extraction. The result from mordanting experiments indicated that as compared to chemical mordants, bio-mordant gives good colour strength both in pre and post mordanting before and after dyeing at optimum conditions. Moreover, 3% of bio-mordant as pre-and 5% as post-mordant give good colour strength, which makes the process more ecofriendly.