Massively parallel C. elegans tracking provides multi-dimensional fingerprints for phenotypic discovery
Journal of neuroscience methods, 2018•Elsevier
Background The nematode worm C. elegans is a model organism widely used for studies of
genetics and of human disease. The health and fitness of the worms can be quantified in
different ways, such as by measuring their bending frequency, speed or lifespan. Manual
assays, however, are time consuming and limited in their scope providing a strong
motivation for automation. New method We describe the development and application of an
advanced machine vision system for characterising the behaviour of C. elegans, the Wide …
genetics and of human disease. The health and fitness of the worms can be quantified in
different ways, such as by measuring their bending frequency, speed or lifespan. Manual
assays, however, are time consuming and limited in their scope providing a strong
motivation for automation. New method We describe the development and application of an
advanced machine vision system for characterising the behaviour of C. elegans, the Wide …
Background
The nematode worm C. elegans is a model organism widely used for studies of genetics and of human disease. The health and fitness of the worms can be quantified in different ways, such as by measuring their bending frequency, speed or lifespan. Manual assays, however, are time consuming and limited in their scope providing a strong motivation for automation.
New method
We describe the development and application of an advanced machine vision system for characterising the behaviour of C. elegans, the Wide Field-of-View Nematode Tracking Platform (WF-NTP), which enables massively parallel data acquisition and automated multi-parameter behavioural profiling of thousands of worms simultaneously.
Results
We screened more than a million worms from several established models of neurodegenerative disorders and characterised the effects of potential therapeutic molecules for Alzheimer’s and Parkinson’s diseases. By using very large numbers of animals we show that the sensitivity and reproducibility of behavioural assays is very greatly increased. The results reveal the ability of this platform to detect even subtle phenotypes.
Comparison with existing methods
The WF-NTP method has substantially greater capacity compared to current automated platforms that typically either focus on characterising single worms at high resolution or tracking the properties of populations of less than 50 animals.
Conclusions
The WF-NTP extends significantly the power of existing automated platforms by combining enhanced optical imaging techniques with an advanced software platform. We anticipate that this approach will further extend the scope and utility of C. elegans as a model organism.
Elsevier
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