[PDF][PDF] Massively parallel single-cell genome sequencing enables high-resolution analysis of soil and marine microbiome
To improve our understanding of the environmental microbiome, we developed a single-cell
genome sequencing platform, named SAG-gel, which utilizes gel beads for single-cell
isolation, cell lysis, and whole genome amplification (WGA) for sequencing. SAG-gel
enables serial, parallel and independent reactions of> 100,000 single cells in a single tube,
delivering high-quality genome recovery with storable randomized single-cell genome
libraries. From soil and marine environmental sources, we acquired 734 partial genomes …
genome sequencing platform, named SAG-gel, which utilizes gel beads for single-cell
isolation, cell lysis, and whole genome amplification (WGA) for sequencing. SAG-gel
enables serial, parallel and independent reactions of> 100,000 single cells in a single tube,
delivering high-quality genome recovery with storable randomized single-cell genome
libraries. From soil and marine environmental sources, we acquired 734 partial genomes …
Massively parallel single-cell genome sequencing enables high-resolution analysis of soil and marine microorganisms
To improve our understanding of the environmental microbiome, we developed a single-cell
genome sequencing platform, named SAG-gel, which utilizes gel beads for single-cell
isolation, cell lysis, and whole genome amplification (WGA) for sequencing. SAG-gel
enables serial, parallel and independent reactions of> 100,000 single cells in a single tube,
delivering high-quality genome recovery with storable randomized single-cell genome
libraries. From soil and marine environmental sources, we acquired 734 partial genomes …
genome sequencing platform, named SAG-gel, which utilizes gel beads for single-cell
isolation, cell lysis, and whole genome amplification (WGA) for sequencing. SAG-gel
enables serial, parallel and independent reactions of> 100,000 single cells in a single tube,
delivering high-quality genome recovery with storable randomized single-cell genome
libraries. From soil and marine environmental sources, we acquired 734 partial genomes …
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