Measurement of DNA morphological parameters at highly entangled regime on surfaces

A Calo, P Stoliar, E Bystrenova, F Valle… - The journal of physical …, 2009 - ACS Publications
The journal of physical chemistry B, 2009ACS Publications
The morphology of circular DNA deposited from a solution on the mica surface is analyzed
from the power spectrum density (PSD) of the atomic force microscopy (AFM) images.
Sample morphology is modulated in a broad range of concentration C from isolated
molecules to highly entangled networks. DNA exhibits a multiaffine behavior with two
correlation length scales: the persistence length P which remains constant (≈ 50 nm) within
the C range and the intermolecular distance ξ which exhibits a decay with increasing C …
The morphology of circular DNA deposited from a solution on the mica surface is analyzed from the power spectrum density (PSD) of the atomic force microscopy (AFM) images. Sample morphology is modulated in a broad range of concentration C from isolated molecules to highly entangled networks. DNA exhibits a multiaffine behavior with two correlation length scales: the persistence length P which remains constant (≈50 nm) within the C range and the intermolecular distance ξ which exhibits a decay with increasing C. Applying a diffusion based model in which ξ scales as ξ ≈ D−0.25·C−0.5, we extracted the DNA diffusion coefficient D ≈ 2 × 10−7 cm2/s. This value is consistent with a high-molecular-weight plasmid DNA supercoiled in the solution.
ACS Publications
以上显示的是最相近的搜索结果。 查看全部搜索结果

Google学术搜索按钮

example.edu/paper.pdf
搜索
获取 PDF 文件
引用
References