[HTML][HTML] Metabolic labeling of the bacterial peptidoglycan by functionalized glucosamine
Y Xu, VM Hernández-Rocamora, JH Lorent, R Cox… - Iscience, 2022 - cell.com
N-Acetylglucosamine (GlcNAc) is an essential monosaccharide required in almost all
organisms. Fluorescent labeling of the peptidoglycan (PG) on N-acetylglucosamine has
been poorly explored. Here, we report on the labeling of the PG with a bioorthogonal handle
on the GlcNAc. We developed a facile one-step synthesis of uridine diphosphate N-
azidoacetylglucosamine (UDP-GlcNAz) using the glycosyltransferase OleD, followed by in
vitro incorporation of GlcNAz into the peptidoglycan precursor Lipid II and fluorescent …
organisms. Fluorescent labeling of the peptidoglycan (PG) on N-acetylglucosamine has
been poorly explored. Here, we report on the labeling of the PG with a bioorthogonal handle
on the GlcNAc. We developed a facile one-step synthesis of uridine diphosphate N-
azidoacetylglucosamine (UDP-GlcNAz) using the glycosyltransferase OleD, followed by in
vitro incorporation of GlcNAz into the peptidoglycan precursor Lipid II and fluorescent …
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