Method to determine protein concentration in the protein–nanoparticle conjugates aqueous solution using circular dichroism spectroscopy
Considerable efforts have been made to synthesize and characterize protein–nanoparticle
conjugates (protein–NPs) for their promising applications in bionanotechnology. However,
protein concentration determination in the protein–NPs has so far not been reported. In this
Letter, we present a simple and nondestructive approach to quantify the protein
concentration in the protein–NPs aqueous solution using circular dichroism (CD)
spectroscopy. Carbon dots (∼ 4 nm), gold nanoparticles (∼ 10 nm), and polyethylene glycol …
conjugates (protein–NPs) for their promising applications in bionanotechnology. However,
protein concentration determination in the protein–NPs has so far not been reported. In this
Letter, we present a simple and nondestructive approach to quantify the protein
concentration in the protein–NPs aqueous solution using circular dichroism (CD)
spectroscopy. Carbon dots (∼ 4 nm), gold nanoparticles (∼ 10 nm), and polyethylene glycol …
[引用][C] Method To Determine Protein Concentration in the Protein–Nanoparticle Conjugates Aqueous Solution Using Circular Dichroism Spectroscopy
L Shanghao, P Zhili - 2015
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