MicroRNA-214 suppresses growth, migration and invasion through a novel target, high mobility group AT-hook 1, in human cervical and colorectal cancer cells
KS Chandrasekaran, A Sathyanarayanan… - British journal of …, 2016 - nature.com
British journal of cancer, 2016•nature.com
Background: MicroRNA-214 (miR-214) has been shown to act as a tumour suppressor in
human cervical and colorectal cancer cells. The aim of this study was to experimentally
validate high mobility group AT-hook 1 as a novel target for miR-214-mediated suppression
of growth and motility. Methods: HMGA1 and miR-214 expression levels were estimated in
cervical and colorectal clinical specimens using qPCR. HMGA1 3′ untranslated region
luciferase assays were performed to validate HMGA1 as a target of miR-214. Effect of …
human cervical and colorectal cancer cells. The aim of this study was to experimentally
validate high mobility group AT-hook 1 as a novel target for miR-214-mediated suppression
of growth and motility. Methods: HMGA1 and miR-214 expression levels were estimated in
cervical and colorectal clinical specimens using qPCR. HMGA1 3′ untranslated region
luciferase assays were performed to validate HMGA1 as a target of miR-214. Effect of …
Abstract
Background:
MicroRNA-214 (miR-214) has been shown to act as a tumour suppressor in human cervical and colorectal cancer cells. The aim of this study was to experimentally validate high mobility group AT-hook 1 as a novel target for miR-214-mediated suppression of growth and motility.
Methods:
HMGA1 and miR-214 expression levels were estimated in cervical and colorectal clinical specimens using qPCR. HMGA1 3′ untranslated region luciferase assays were performed to validate HMGA1 as a target of miR-214. Effect of altering the expression of miR-214 or HMGA1 on proliferation, migration and invasion of human cervical and colorectal cancer cells was investigated.
Results:
miR-214 expression was poor while that of HMGA1 was high in cervical and colorectal cancer tissues. miR-214-re-expression or HMGA1 downregulation inhibited proliferation, migration and invasion of cancer cells while miR-214 inhibition had opposite effects. miR-214 was demonstrated to bind to the wild-type 3′ untranslated region of HMGA1 but not with its mutant.
Conclusions:
Low expression of miR-214 concurrent with elevated levels of HMGA1 may contribute to cervical and colorectal cancer progression. miR-214-mediated regulation of HMGA1 is a novel mechanism for its tumour-suppressive actions in human cervical and colorectal cancer cells and opens up avenues for novel therapeutic strategies for these two cancers.
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