A model for water–macromolecular magnetization transfer is presented which addresses the
mechanism of coupling between the hydrogen populations and the extraction of physically
meaningful parameters from experimental magnetization transfer data. Both physical
exchange between bulk-solvent and site-specific hydration-layer hydrogens and
intermolecular magnetic dipolar coupling between these specific hydration-layer-solvent
and macromolecular hydrogens are explicitly included, leading to a three-pool model for …