As environmental concerns grow, flotation researches, particularly for the recovery of fine-grained ores, encourage "bioflotation" studies, in which biological origin alternatives are used instead of traditional flotation reactives. While bioflotation applications starting from the pyrite mineral have spread to many carbonate and oxide minerals over time, and biomaterials used as a bioreagent have diversified from the microorganism itself to its metabolites and even cell components. In this article, the use of surfactin derived from Bacillus subtilis as a bio-collector in the flotation of magnesite was investigated. The results of bioflotation studies were compared to those of oleate, traditional magnesite collector. Moreover, process models were created with statistical design methods, and the verification results of the optimization studies using model datas showed that these models were statistically strong.