Molecular Assessment of Genetic Stability Using CDDP and DNA-barcoding Assays in Long-term Micropropagated Rose Plant.

SD ED, AO Attia, A Ismail, SS Alotaibi - Pakistan Journal of …, 2020 - europepmc.org
Pakistan Journal of Biological Sciences: PJBS, 2020europepmc.org
Materials and methods Ten combinations of CDDP PCR primers were employed and the
rpoC1 gene region was sequenced for mother plant (control) and micropropagated plantlets
of Taif rose plant. Results Based on CDDP data, phylogenetic divergence indicated that the
distinct specimens of Taif rose micro-propagated plantlets and control were genetically
differentiated by a difference of 1% of genetic dissimilarity. Phylogenetic tree which
developed using rpoC1 DNA showed that rpoC1 DNA sequencing discovered a genetic …
Materials and methods
Ten combinations of CDDP PCR primers were employed and the rpoC1 gene region was sequenced for mother plant (control) and micropropagated plantlets of Taif rose plant.
Results
Based on CDDP data, phylogenetic divergence indicated that the distinct specimens of Taif rose micro-propagated plantlets and control were genetically differentiated by a difference of 1% of genetic dissimilarity. Phylogenetic tree which developed using rpoC1 DNA showed that rpoC1 DNA sequencing discovered a genetic difference between the control and micro-propagated plantlets of Taif rose.
Conclusion
Furthermore, CDDP and DNA barcoding using rpoC1 gene have demonstrated their usefulness in investigating the genetic history of Rosa species and their ability to explore genetic mutation.
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