Correspondence being maintained by an avian reservoir host helps increase virus
population size that decreases founder effects and genetic drifts which may explain their
sequence conservation16-19. One does not know the migratory patterns of both passerines
and shorebirds to encompass much of Southeast Asia ranging from Yellow Sea and South
China sea across Philippines and Indonesia to Australia. Relevance of such avian
transmission is very much a likely phenomenon to the districts of Andhra Pradesh becasue …

Methods: RT-PCR was used for screening of suspected blood samples. Primers were
designed to amplify partial E1 gene and the amplified fragment was cloned and sequenced.
The sequence was analyzed and compared with other geographical isolates to find the
phylogenetic relationship. Results: The sequence was submitted to the Gen bank DNA
database (accession DQ888620). Comparative nucleotide homology analysis of the AP Ra-
CTR isolate with the other isolates revealed 94.7±3.6 per cent of homology of CHIKAPRa …