Lake Tempe is one of the tectonic lakes in South Sulawesi and it is a source of freshwater shrimp fisheries such as Macrobrachium idea. The local name of this shrimp is urang salo or udang puce. This research aimed to analyze the DNA barcoding of M. idae from Tempe Lake, South Sulawesi, Indonesia. Polymerase Chain Reaction (PCR) primers used were LCO1490 (5'-GGTCAACAA ATCATAAAGATATTGG-3') and HCO2198 (5'-TAAACTTCAGGGTGACC AAAAAATCA-39). Data were collected using a local tool known as Bubu, while genomic DNA was isolated to determine PCR, electrophoresis, gel purification, and DNA sequencing. Phylogenetic analysis using the Neighbor-Joining Method and sequencing Basic Local Alignment Search Tool (BLAST) was based on the NCBI database. The results showed that the BLAST analysis of M. idae was based on data from NCBI obtained 93.37-100% with a DNA fragment size of 684 bp. Furthermore, the Neighbor-Joining method showed that M. idae is different from Macrobrachium idella, Macrobrachium meridionalis and Macrobrachium rosenbergii.