Molecular tools for pathway engineering in Saccharomyces cerevisiae
PB Besada-Lombana, TL McTaggart… - Current opinion in …, 2018 - Elsevier
PB Besada-Lombana, TL McTaggart, NA Da Silva
Current opinion in biotechnology, 2018•ElsevierHighlights•Advances in CRISPR-Cas9 aid tool development and multiplexed yeast
engineering.•New catalogue of promoters, trans-acting factors and terminators advance
control.•Novel terminators, RNAi and riboswitches enhance final mRNA regulation.•Protein
tags allow improved control over protein turnover and spatial localization.•Developments in
small molecule sensing strategies optimize pathway engineering.Molecular tools for the
regulation of protein expression in Saccharomyces cerevisiae have contributed to rapid …
engineering.•New catalogue of promoters, trans-acting factors and terminators advance
control.•Novel terminators, RNAi and riboswitches enhance final mRNA regulation.•Protein
tags allow improved control over protein turnover and spatial localization.•Developments in
small molecule sensing strategies optimize pathway engineering.Molecular tools for the
regulation of protein expression in Saccharomyces cerevisiae have contributed to rapid …
Highlights
- Advances in CRISPR-Cas9 aid tool development and multiplexed yeast engineering.
- New catalogue of promoters, trans-acting factors and terminators advance control.
- Novel terminators, RNAi and riboswitches enhance final mRNA regulation.
- Protein tags allow improved control over protein turnover and spatial localization.
- Developments in small molecule sensing strategies optimize pathway engineering.
Molecular tools for the regulation of protein expression in Saccharomyces cerevisiae have contributed to rapid advances in pathway engineering for this yeast. This review considers new and enhanced additions to this toolbox, focusing on experimental approaches to modulate enzyme synthesis and enzyme fate. Methods for genome engineering, regulation of transcription, post-translational protein localization, and combinatorial screening and sensing in S. cerevisiae are highlighted, and promising new approaches are introduced.
Elsevier
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