Genetically encoded probes have become powerful tools for non-invasive monitoring of
ions, distributions of proteins and the migration and formation of cellular components. We
describe the functional expression of two molecular probes for non-invasive fluorescent
monitoring of intracellular Cl ([Cl] i) and the functioning of glycine receptor (GlyR) channels.
The first probe is a recently developed cyan fluorescent protein–yellow fluorescent protein-
based construct, termed Cl-Sensor, with relatively high sensitivity to Cl (K app∼ 30 mM). In …

Genetically encoded probes have become powerful tools for non-invasive monitoring of
ions, distributions of proteins and the migration and formation of cellular components. We
describe the functional expression of two molecular probes for non-invasive fluorescent
monitoring of intracellular Cl ([Cl] i) and the functioning of glycine receptor (GlyR) channels.
The first probe is a recently developed cyan fluorescent protein–yellow fluorescent protein-
based construct, termed Cl-Sensor, with relatively high sensitivity to Cl (Kappw30 mM). In …