Metaproteomics, the study of protein expression in microbial communities, is a versatile tool
for environmental microbiology. Achieving sufficiently high metaproteome coverage to
obtain a comprehensive picture of the activities and interactions in microbial communities is
one of the current challenges in metaproteomics. An essential step to maximize the number
of identified proteins is peptide separation via liquid chromatography (LC) prior to mass
spectrometry (MS). Thorough optimization and comparison of LC methods for …