Because it avoids the in-focus photodamage and phototoxicity problem of two-photon-
fluorescence excitation, multiharmonic-generation biopsy based on a 1200–1300-nm light
source could provide a truly noninvasive and highly penetrative optical sectioning of skin.
We study multiharmonic-generation biopsy of fixed mouse skin. Our preliminary study
suggests that this technique could provide submicrometer-resolution deep-tissue
noninvasive biopsy images in skin without the use of fluorescence and exogenous markers.

Multiharmonic generation biopsy of skin | IEEE Conference Publication | IEEE Xplore
Multiharmonic generation biopsy of skin Abstract: Avoiding the on-focus photodamage and
phototoxicity problem of two-photon-fluorescence excitation, harmonic generation biopsy
based on a /spl sim/1300 nm light source provides a truly noninvasive and highly
penetrative optical sectioning of skin. …