Abstract N-Acetylglucosamine-6-O-sulfotransferase (GlcNAc6ST) catalyzes the transfer of
sulfate from 3′-phosphoadenosine 5′-phosphosulfate to the C-6 position of non-reducing
GlcNAc. Human GlcNAc6ST-1 was expressed as a fusion protein with protein A in an insect
cell line (Tn 5 cells) using the baculovirus system. The recombinant enzyme was purified to
homogeneity by IgG Sepharose column chromatography. The substrate specificity and the
kinetic properties of the enzyme were similar to those of the enzyme expressed in the …

N-acetylglucosamine-6-O-sulfotransferase-1 : production in the baculovirus system and its
application to synthesis of a sulfated oligosaccharide and to modification of
oligosaccharides in fibrinogen | CiNii Research … N-acetylglucosamine-6-O-sulfotransferase-1
: production in the baculovirus system and its application to synthesis of a sulfated
oligosaccharide and to modification of oligosaccharides in fibrinogen … タイトル "N-acetylglucosamine-6-O-sulfotransferase-1
: production in the baculovirus system and its application to synthesis of a sulfated …