Nanoflow electrospinning serial femtosecond crystallography
Acta Crystallographica Section D: Biological Crystallography, 2012•journals.iucr.org
An electrospun liquid microjet has been developed that delivers protein microcrystal
suspensions at flow rates of 0.14–3.1 µl min− 1 to perform serial femtosecond
crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl
min− 1 and diffracted to beyond 4 Å resolution, producing 14 000 indexable diffraction
patterns, or four per second, from 140 µg of protein. Nanoflow electrospinning extends SFX
to biological samples that necessitate minimal sample consumption.
suspensions at flow rates of 0.14–3.1 µl min− 1 to perform serial femtosecond
crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl
min− 1 and diffracted to beyond 4 Å resolution, producing 14 000 indexable diffraction
patterns, or four per second, from 140 µg of protein. Nanoflow electrospinning extends SFX
to biological samples that necessitate minimal sample consumption.
An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14–3.1 µl min−1 to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min−1 and diffracted to beyond 4 Å resolution, producing 14 000 indexable diffraction patterns, or four per second, from 140 µg of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.
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