Amidst countless challenges, such as the need to see the real importance of mechanisms for maintaining species diversity (Chesson 2000), we must invest strong efforts to collect biological data on Neotropical gradients. Despite the large number of variables and mechanisms deriving from these gradients, the theory of ecological communities can be used to understand the processes responsible for generating and maintaining diversity in biological communities (Graham et al. 2014; Vellend 2010). With the current biodiversity crisis, especially caused by anthropic actions, we must explore the evolution of analyses and the growth (in quality and quantity) of databases to allow us to use a range of explanatory variables to calculate complex properties of biological communities (Baselga and Orme 2012) and reduce sampling problems, such as temporal pseudo-replication (Crawley 2013).