New generation of bioorthogonally applicable fluorogenic dyes with visible excitations and large stokes shifts
A Herner, G Estrada Girona, I Nikic, M Kállay… - Bioconjugate …, 2014 - ACS Publications
Bioconjugate chemistry, 2014•ACS Publications
Synthesis of a set of new, azide bearing, biorthogonally applicable fluorogenic dyes with
large Stokes shifts is presented herein. To assess the fluorogenic performance of these new
dyes we have labeled a genetically modulated, cyclooctyne-bearing protein in lysate
medium. Studies showed that the labels produce specific signal with minimal background
fluorescence. We also provide theoretical insights into the design of such fluorogenic labels.
large Stokes shifts is presented herein. To assess the fluorogenic performance of these new
dyes we have labeled a genetically modulated, cyclooctyne-bearing protein in lysate
medium. Studies showed that the labels produce specific signal with minimal background
fluorescence. We also provide theoretical insights into the design of such fluorogenic labels.
Synthesis of a set of new, azide bearing, biorthogonally applicable fluorogenic dyes with large Stokes shifts is presented herein. To assess the fluorogenic performance of these new dyes we have labeled a genetically modulated, cyclooctyne-bearing protein in lysate medium. Studies showed that the labels produce specific signal with minimal background fluorescence. We also provide theoretical insights into the design of such fluorogenic labels.
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