Profound metabolic changes are characteristic of macrophages during classical activation
and have been implicated in this phenotype. Here we demonstrate that nitric oxide (NO)
produced by murine macrophages is responsible for TCA cycle alterations and citrate
accumulation associated with polarization. 13C tracing and mitochondrial respiration
experiments map NO-mediated suppression of metabolism to mitochondrial aconitase
(ACO2). Moreover, we find that inflammatory macrophages reroute pyruvate away from …