No evidence for persistence of BCR-ABL–positive cells in patients in molecular remission after conventional allogeneic transplantation for chronic myeloid leukemia
M Deininger, T Lehmann, R Krahl… - Blood, The Journal …, 2000 - ashpublications.org
M Deininger, T Lehmann, R Krahl, E Hennig, C Müller, D Niederwieser
Blood, The Journal of the American Society of Hematology, 2000•ashpublications.orgBlood ASH 96/2 treatment with BMT, and serial samples were analyzed for levels of
genomic and mRNA BCR-ABL using DNA-PCR with patientspecific primers and RT-PCR,
respectively. Eight patients became RT-PCR–negative after BMT. All these patients also
became DNA-PCR–negative either concurrently or, in one case, shortly after. Again, this
study was not consistent with the hypothesis of common, persistent genomic BCR-ABL
rearrangement without mRNA expression. In conclusion, we believe that our data …
genomic and mRNA BCR-ABL using DNA-PCR with patientspecific primers and RT-PCR,
respectively. Eight patients became RT-PCR–negative after BMT. All these patients also
became DNA-PCR–negative either concurrently or, in one case, shortly after. Again, this
study was not consistent with the hypothesis of common, persistent genomic BCR-ABL
rearrangement without mRNA expression. In conclusion, we believe that our data …
Blood ASH 96/2 treatment with BMT, and serial samples were analyzed for levels of genomic and mRNA BCR-ABL using DNA-PCR with patientspecific primers and RT-PCR, respectively. Eight patients became RT-PCR–negative after BMT. All these patients also became DNA-PCR–negative either concurrently or, in one case, shortly after. Again, this study was not consistent with the hypothesis of common, persistent genomic BCR-ABL rearrangement without mRNA expression.
In conclusion, we believe that our data demonstrate concordance of BCR-ABL mRNA and DNA levels after BMT for CML. But the results reported by Chomel et al deserve further attention because they would, if confirmed by others, require radical revision of our concepts of ‘‘minimal residual disease’’after allografting for CML.
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