One-step cloning and chromosomal integration of DNA
ACS synthetic biology, 2013•ACS Publications
We describe “clonetegration”, a method for integrating DNA into prokaryotic chromosomes
that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared
to existing techniques, clonetegration drastically decreases the time and effort needed for
integration of single or multiple DNA fragments. Additionally, clonetegration facilitates
cloning and expression of genetic elements that are impossible to propagate within typical
multicopy plasmids.
that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared
to existing techniques, clonetegration drastically decreases the time and effort needed for
integration of single or multiple DNA fragments. Additionally, clonetegration facilitates
cloning and expression of genetic elements that are impossible to propagate within typical
multicopy plasmids.
We describe “clonetegration”, a method for integrating DNA into prokaryotic chromosomes that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of single or multiple DNA fragments. Additionally, clonetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids.
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