Lanka is placed as one of the top manufactures of the world which also contributes more than 5% of global demand. Natural rubber latex is one of the most important polymer materials which is widely used for manufacturing of gloves. Centrifuged latex contains 60% dry rubber content is used for glove manufacturing process. Addition of fillers may have a potential to improve the stiffness like inferior properties of Natural rubber and simultaneously reduces the cost of production by replacing part of rubber by the fillers. Among the types of fillers used with Natural rubber, calcium carbonate (CaCO3) which generally categorizes under non-reinforcing filler is widely used in glove industry due to its availability and the fewer prices. Optimization of calcium carbonate amount in rubber matrices is essential to keep the required limits of specification of physio-mechanical properties in gloves. In this study CaCO3 filled Natural rubber latex disposable glove samples were successfully prepared by varying the CaCO3 loading level from 20 phr to 45 phr at 5 phr intervals. Tensile strength, elongation at break and force at break were investigated as per the physio-mechanical properties of each sample at aged (after aging) and unaged (before aging) conditions. The experiment was conducted in complete randomized design with 3 replicates per each sample. Thirty (30phr) CaCO3 loading level was indicated the best tensile strength of unaged and aged above 17 N/mm2, elongation at break of aged and unaged samples above 700% and force at break of unaged samples above 9N and aged samples above 6.3 N. The results of the statistical analysis indicate that the 30 phr CaCO3 loading level can be considered as the optimum CaCO3 loading level compared to the specifications of the physio-mechanical properties of natural rubber latex disposable gloves.